Figure 5.

AGE-RAGE suppresses Il6 mediated-phosphorylation of Stat3 and up-regulation of Pim1 in hepatocytes. A) Ex vivo, hepatocytes were isolated from WT or RAGE-null mice 2 h after 85% resection, and the mRNA transcripts levels of Pim1 were determined by real-time PCR. Data are means ± se; n = 3. *P < 0.05. B) Hepatocytes were isolated from WT or RAGE-null mice and stimulated with AGE, Il6, or AGE plus Il6 as described. Immunoblotting was performed with anti-phospho-Stat3 IgG, followed by anti-Gapdh IgG; n = 3 mice/condition. C) Hepatocytes were isolated from RAGE-null mice and stimulated by AGE and Il6, and an Il6 receptor antagonist (anti-Il6r). Immunoblotting was performed with anti-phospho-Stat3 IgG or anti-Pim1 IgG, followed by anti-Gapdh IgG. Illustrated bands are representative of n = 3 mice/condition.