Figure 6. Depletion of LSDP5 increased fatty acid β-oxidation and the number of mitochondria.

AML12 cells were infected with adenovirus carrying LSDP5 siRNA (MOI = 90) or an adenovirus carrying control siRNA for 24 h. (A) β-oxidation of [³H]-oleate in si-control or si-LSDP5 cells. Data are presented as the mean±SEM (n = 4), ^*P<0.05. (B) The mRNA levels of CPT1α, Sdha, Cox4 and Cox7a1 were assessed using real-time PCR. The relative mRNA level in the control group was designated as 1.0. Data are presented as the mean±SEM (n = 5), ^*P<0.05. (C) Mitochondria in hepatocytes containing control-siRNA or LSDP5-siRNA were stained with MitoTracker Red. Nuclei were labeled with Hoechst 33258. Scale bar = 5 µm. ^*P<0.05. (D) Changes in the copy number of mtDNA were assessed with real-time PCR. The relative amount of mtDNA was calculated as the normalized ratio of NADH dehydrogenase subunit I/lipoprotein lipase, and the relative mtDNA copy number in the control group was designated as 1.0. Data are presented as the mean±SEM (n = 4), ^* P<0.05. Data in this figure were analyzed with paired Student’s t tests.