Figure 2. Over-expression of synaptogyrin constructs.

(A) Relative over-expression yields of the synaptogyrin constructs in different expression strains determined via Western blot after crude membrane preparation using a HRP-conjugated His probe. Construct with the highest Western blot intensity was set to 100 percent. Error bars are derived from three independent Western blots to minimise errors caused by inefficient transfer of IMPs from the SDS gels onto the membrane. (B) Final optical density of the synaptogyrin constructs in the corresponding expression strain prior to harvest.