Figure 3. Lemo21(DE3) as over-expression host for synaptogyrin.

(A) Over-expression yields of synaptogyrin 1b construct 5 as quantified by Western blot of crude membranes in different over-expression strains. The different rhamnose concentrations used with the Lemo21(DE3) strain are indicated. Expression strain with the highest expression level was set to 100 percent Western blot intensity. Lower panel shows the final OD_{600 nm} values reached before harvest. (B) Same as in (A) but data are shown for the synaptogyrin 2 construct. (C) Over-expression results for synaptogyrin 3 as described for (A). Error bars are derived from three independent Western blot analyses.