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. 2012 Jun 1;7(6):e38244. doi: 10.1371/journal.pone.0038244

Figure 4. Detergent screening of synaptogyrin constructs.

Figure 4

(A) Solubilisation efficiencies of synaptogyrin 1 constructs in different detergents (SDS, FC12, DDM, DM, Triton X-100, LDAO and C12E8). Crude membranes solubilised in SDS were used as a reference and obtained Western blot signal corresponds to 100 percent solubilisation efficiency. Only membranes with the highest expression levels for a particular constructs were used for this solubilisation screen: (−) syn1b c1 (Rosetta2(DE3)); (−) syn1b c2 (Rosetta2(DE3)); (−) syn1b c4 (Rosetta2(DE3)); (−) syn1b c5 (Rosetta2(DE3)); (−) syn1b c5 (Lemo21(DE3), 250 µM rhamnose). (B) Comparison of solubilisation efficiencies for the synaptogyrin 2 protein expressed in the (−) Rosetta2(DE3) strain or (−) Lemo21(DE3) (100 µM rhamnose). (C) Relative solubilisation efficiencies of the synaptogyrin 3 protein expressed in the (−) Rosetta2(DE3) strain or (−) Lemo21(DE3) (0 µM rhamnose). Error bars are derived from three independent Western blot analyses.