Skip to main content
. 2012 Jun 1;7(6):e38244. doi: 10.1371/journal.pone.0038244

Figure 5. Analytical gel filtration (AGF) screening of synaptogyrin.

Figure 5

(A) Analytical gel filtration of different synaptogyrin 1 constructs after solubilisation in DDM and subsequent IMAC purification. The void volume is indicated. Synaptogyrin 1 constructs elute as monodisperse peaks from the gel filtration column indicative for high homogeneity: (−) syn1b c1; (−) syn1b c2; (−) syn1b c4; (−) syn1b c5. (B) Synaptogyrin 1b construct 5 remains stable and elutes as symmetrical peak from the analytical gel filtration column in different detergents ((−) DDM; (−) UDM; (−) DM; (−) LDAO; (−) OG).