Figure 7. Crosslinking experiments of synaptogyrin 1 in the presence of different bifunctional crosslinkers support an oligomeric architecture.

(A) Crosslinking of DDM solubilised and purified synaptogyrin 1 in the presence of 0.25 mM DSS. (B) Higher oligomeric species of synaptogyrin 1 are also obtained when the crosslinking reaction (2 mM DMS) is performed on crude membranes. (C) Similar conclusions result from crosslinking experiments of Triton X-100 solubilised and purified material in the presence of 1 mM glutaraldehyde. Crosslinking reactions were stopped after the given time points by adding 200 mM of Tris solution and subsequent boiling of the sample.