Figure 3.

Simulation of chemical concentration gradients with dcEF application in the microfluidic device. (a) Simulated normalized CCL19 gradient in the main channel of the microfluidic device when a 10 V electrical potential difference is applied to the device through the 2 electrode wells. (b) Enlarged view of the CCL19 gradient in the boxed region in the main channel as indicated in (a) (∼7 mm downstream of the main channel). (c) Plot of simulated gradient across the main channel width in the region as in (b) with (grey) or without (black) the applied dcEF. The simulation results show that the gradient profile is not significantly affected by the applied dcEF. The p value of the F test comparing the gradient profiles is 0.82.