Figure 4.

Chemotaxis of activated T cells in the microfluidic device. Angular histograms of cell migration angles in the control condition (medium only) or in a 100 nM CCL19 gradient are showed in (a) and (b), respectively. The rose diagrams show the distribution of migration angles of all cells analyzed from multiple independent experiments for each condition. The migration angles were calculated from x-y coordinates at the beginning and the end of the cell tracks and were grouped in 20° intervals, with the radius of each wedge indicating the cell number (i.e., the radius of each circle indicates the cell number with the increment of one). (c) O.I. and speed of cells in the control condition or in a 100 nM CCL19 gradient. The values are presented as the average ± SEM. The results show the effectiveness of the developed microfluidic device for analyzing cell chemotaxis in single chemokine gradients.