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. 2012 May 16;6(2):024121–024121-13. doi: 10.1063/1.4718721

Figure 6.

Figure 6

T cell migration in competing CCL19 gradients and dcEF in the microfluidic device. (a) Migration tracks of 6 cells from a representative experiment with 2 cells (grey) migrating toward the 100 nM CCL19 gradient (left) and 4 cells (black) migrating toward the cathode of the applied dcEF (10 V across the device with the cathode on the right). (b) Angular histogram shows the distribution of migration angles of all cells analyzed from multiple independent experiments. The migration angles were calculated from x-y coordinates at the beginning and the end of the cell tracks and were grouped in 20° intervals, with the radius of each wedge indicating the cell number (i.e., the radius of each circle indicates the cell number with the increment of one). (c) O.I. and speed of T cells in single CCL19 gradient, single dcEF or competing CCL19 gradient and dcEF. The values are presented as the average ± SEM. The results show the stronger cell migration toward the cathode of the applied dcEF in the presence of a competing CCL19 gradient.