Figure 2. Kv1.2 and Kv2.1 are highly phosphorylated in rat brain.
Phosphorylation in Kv1.2 and Kv2.1 channels causes altered electrophoretic mobility on SDS-PAGE. Phosphorylation is revealed by differences in electrophoretic migration of these proteins in samples of rat brain membranes (RBM) analyzed by immunoblot without (-) or with (+) prior alkaline phosphatase (AP) treatment of the RBM.
