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. 2012 Apr 6;287(23):18872–18887. doi: 10.1074/jbc.M112.355958

FIGURE 5.

FIGURE 5.

PTAA inhibits prion accumulation in RML6-infected slice cultures from different time points. A, MPA of homogenates from RML6-infected slices harvested at days 7, 19, 21, 28, 35, and 42 DIV. PTAA was administered at a concentration of 10 μg ml−1 at the time of a fresh medium change from days 7, 19, 21, 28, or 35 DIV. Slice cultures were harvested at 42 DIV and analyzed for PrP aggregates. Each dot represents the average value of technical triplicates for one slice culture homogenate. Error bars represent the mean ± S.E. of biological duplicates. All data were corrected for noninfectious brain homogenates obtained from CD1 mice as negative control (mock) and represented as the ratio between relative light units (RLU) and protein concentration for each sample. p values are shown in supplemental Table S9. B, sandwich ELISA of the same homogenates as in A analyzed for PrPSc. The data are represented as biological triplicates. The signal for the negative control (mock) is shown as a single replica. C, sandwich ELISA of the same homogenates as in A analyzed for PrPC. The ELISA was developed by chemiluminescence. The detection limit of 600 pg ml−1 (dashed line) was determined as the mean background levels plus three times S.D.