FIGURE 4.
Interaction of RAGE with cell-surface CS and HS by flow cytometry. The binding potential of RAGE was assessed by immunofluorescence flow cytometry toward LLC cells treated with no enzymes (A), chondroitinase (CSase, B), or with a mixture of heparinases I and III (HSase; C). Each cell line was treated with a RAGE/Fc chimera followed by Alexa Fluor 488-conjugated protein G. Green and red histograms represent the RAGE binding and the background fluorescence, respectively.