TABLE 1.
Kinetic parameters for the interaction of RAGE with immobilized CS isoforms, HS, and heparin
The ka, kd, and Kd values were determined using a 1:1 Langmuir binding model with mass transfer as described under “Experimental Procedures.” The value for each CS variant, HS, and heparin preparation were expressed as the mean ± S.E. of five different concentrations. Note that the Kd values of CS-A and CS-C could not be determined due to the weak interaction with RAGE (Fig. 2).
| Immobilized GAG | ka | kd | Kd |
|---|---|---|---|
| m−1s−1 | s−1 | nm | |
| CS-B (DS) from porcine skin | (9.4 ± 3.6) × 104 | (1.9 ± 0.02) × 10−2 | 357 ± 136 |
| CS-D from shark fin cartilage | (1.5 ± 0.7) × 105 | (1.6 ± 0.03) × 10−2 | 306 ± 163 |
| CS-E from squid cartilage | (2.8 ± 0.1) × 104 | (5.4 ± 1.9) × 10−6 | 0.19 ± 0.06 |
| HS from bovine kidney | (2.5 ± 0.5) × 104 | (4.5 ± 0.5) × 10−3 | 203 ± 48 |
| HS from porcine intestine | (2.4 ± 0.4) × 104 | (1.5 ± 1.2) × 10−5 | 0.6 ± 0.4 |
| Heparin from porcine intestine | (1.6 ± 0.3) × 104 | (5.0 ± 3.0) × 10−4 | 3.1 ± 1.8 |