FIGURE 6.

Cn delays V-ATPase-dependent cell death. Cultures of wild-type yeast cells were exposed to tunicamycin at 0 h with additions of FK506 (FK) or FK506 plus concanamycin (FK+Con) at 0 h (black), 1 h (red), 2 h (green), or 3 h (blue). A and B, the frequencies of cell death in triplicate cultures were measured at the indicated times by staining with PI and flow cytometry and plotted directly as percentage of dead (A) or plotted after determining the percentage of the population that was rescued by the addition of concanamycin (B), which was calculated by subtraction of the values obtained in the FK506 plus concanamycin cultures from those shown in A. Error bars indicate ±S.D.