FIGURE 8.

Nonvacuolar localization of ROS. Wild-type yeast cells were stained with FM4-64 and subsequently exposed to tunicamycin plus FK506. After 4 h of incubation, cells were stained with dihydro-DCFDA and imaged by confocal microscopy for vacuole membrane architecture (FM4-64) and ROS accumulation (dihydro-DCFDA). The image shows three live cells, two of which are representative of cells that stain positive for ROS. Fluorescence products of dihydro-DCFDA were almost always excluded from the region of the cell containing vacuoles.