FIGURE 1.

Effects of oligonucleotide length on Gp59-dependent fluorescence changes of Gp32F. Fluorescence measurements were performed as described under “Experimental Procedures.” Buffer contained the following components: 20 mm Tris acetate, pH 7.4, 10 mm magnesium acetate, 90 mm potassium acetate, and 1 mm DTT. Fluorescence emission scans monitored helicase loading complex formation at high binding density on each of the following homopolymeric oligonucleotides: dT₇₀ (A), dT₄₀ (B), dT₂₀ (C), and dT₁₂ (D). Solid line, 100 nm Gp32F alone; dotted line, 100 nm Gp32F + 700 nm oligonucleotide; dashed line, 100 nm Gp32F + 700 nm oligonucleotide + 100 nm Gp59.