FIGURE 2.

Effects of M13mp18 ssDNA and Gp59 on fluorescence of Gp32F at low binding density. Fluorescence measurements were performed as described under “Experimental Procedures.” Buffer contained the following components: 20 mm Tris acetate, pH 7.4, 10 mm magnesium acetate, 90 mm potassium acetate, and 1 mm DTT. A, solid line, 30 nm Gp32F alone; dotted line, 30 nm Gp32F + 3 μm M13mp18 ssDNA; dashed line, 30 nm Gp32F + 3 μm M13mp18 ssDNA + 30 nm Gp59. B, solid line, 100 nm Gp32F alone; dashed line,100 nm Gp32F + 100 nm Gp59.