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. 2012 Apr 13;287(23):19094–19104. doi: 10.1074/jbc.M111.322933

FIGURE 1.

FIGURE 1.

Homodimerization of Bnip3 is essential for induction of autophagy. A, HeLa cells overexpressing Bnip3 or Bnip3 mutants were scored for the presence of GFP-LC3-positive autophagosomes. Bnip3, but not Bnip3ΔTM, significantly increased the number of autophagosomes/cell compared with β-gal-infected cells. *, p < 0.05. n = 3. B, Bnip3, but not Bnip3ΔTM, significantly increased the number of cells positive for autophagy compared with β-gal-infected cells. *, p < 0.05. n = 3. Shown is a representative Western blot analysis for endogenous LC3I/II. C, Bnip3, but not the homodimerization-deficient mutants, significantly increased the number of GFP-LC3 positive autophagosomes/cell compared with Myc-transfected control cells. *, p < 0.05. n = 3. D, Bnip3, but not the homodimerization-deficient mutants, induced significant autophagy compared with Myc-transfected control cells. *, p < 0.05. n = 4. Representative Western blot analysis for endogenous LC3I/II. E, the presence of GABARAP-GFP-positive autophagosomes in HeLa cells overexpressing Bnip3. Bnip3, but not the mutants, significantly increased the number of GABARAP-GFP-positive autophagosomes/cell compared with Myc-transfected control cells. *, p < 0.05. n = 3. Representative images are shown. F, Bnip3, but not the mutants, induced autophagy in a significant number of cell compared with Myc-transfected control cells. *, p < 0.05. n = 4.