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. 2012 Apr 13;287(23):19094–19104. doi: 10.1074/jbc.M111.322933

FIGURE 5.

FIGURE 5.

Bnip3 induces autophagy from both the mitochondria and the ER. A, representative images of HeLa cells transfected with vector, Bnip3, Bnip3Acta, or Bnip3cb5 plus GFP-LC3. B, quantitation of the number of GFP-LC3-positive autophagosomes per cell. n = 5. *, **, and ***, p < 0.05 versus Myc. C, quantitation of cells with activated autophagy. Bnip3, Bnip3Acta, and Bnip3cb5 significantly activated autophagy in HeLa cells compared with Myc cells. Data are mean ± S.E. n = 3. *, **, and ***, p < 0.05 versus Myc. D, Bnip3Acta and Bnip3cb5 coimmunoprecipitate (IP) with GFP-LC3 in HeLa cells. E, isolation of autophagosomes from HeLa cells using anti-GFP-linked to magnetic beads. Western blot analysis of isolated autophagosomes showed an increase in the number of autophagosomes and the presence of mitochondria and ER proteins in the autophagosomes in cells overexpressing Bnip3. Results are representative of three independent experiments.