FIGURE 4.

Overexpression of KLF15 increases expression of podocyte differentiation markers in both control and HIV-1-infected cells. A, murine podocytes were transiently transfected with control vector or KLF15 construct, and protein was extracted for Western blot. Cells were collected 24 h after transfection for determining mRNA levels of podocyte differentiation markers by real time PCR. Synaptopodin (B), podocin (C), and nephrin (D) were measured in control and HIV-1-infected cells with and without KLF15 overexpression (n = 3, *, p < 0.05 compared with the control cells; n = 3, **, p < 0.05 compared between cells with or without KLF15 overexpression).