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. 2012 Apr 6;287(23):19136–19147. doi: 10.1074/jbc.M111.323048

FIGURE 2.

FIGURE 2.

Native rabbit cardiac muscle contains cTnI phosphorylated at Ser-150. A, Western blot of two-dimensional isoelectric focusing-separated rabbit ventricular myofibrils probed with the pTnI 150 antibody identified three cTnI species as containing Ser-150 phosphorylation (P1, P2, and P3). B, sequential probing of the same membrane with a total cTnI antibody identified four cTnI species. Alignment of the two membranes demonstrates the three most acidic, phosphorylated cTnI species were detected by the pTnI 150 antibody as containing Ser-150 phosphorylation without detection of the non-phosphorylated, basic species (U).