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. 2012 Apr 19;287(23):19171–19176. doi: 10.1074/jbc.M112.359315

FIGURE 3.

FIGURE 3.

Fibrin and chloride ions modulate human Glu-Pg activation by SUPA. A, effect of chloride on Pg activation. Human Glu-Pg (100 nm) was mixed with HEPES buffer containing various amounts of NaCl (0–150 mm) and 500 μm S-2251. The total ionic strength of the buffers was maintained at 150 mm by the addition of NaOAc to buffers with chloride concentrations <150 mm. After incubation at 37 °C for 10 min, SUPA (40 nm) was added at t = 0, and the absorbance at 405 nm was monitored to detect the generation of plasmin. Results are representative of two experiments. B and C, fibrin fragments enhance Glu-Pg activation by SUPA. Human Glu-Pg (100 nm) was added to Tris/NaCl buffer containing 500 μm S-2251 at 37 °C in the absence (control (Ctrl)) or presence of varying concentrations of CNBr-fibrin fragments, fibrinogen, or BSA. After 10 min, Pg activation was initiated by the addition of 0 (B) or 20 (C) nm SUPA, and the absorbance at 405 nm was monitored. The results shown are representative of two independent experiments.