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. 2012 Apr 19;287(23):19171–19176. doi: 10.1074/jbc.M112.359315

FIGURE 4.

FIGURE 4.

Analysis of human Glu-Pg activation by SUPA in presence of fibrin. Human Glu-Pg (500 nm) was mixed with 40 nm SUPA in Tris/NaCl buffer in the absence or presence of 0.1 mg/ml CNBr-fibrin fragments for 0.5 h at 37 °C. Samples were collected, reduced, and analyzed by SDS-PAGE. Lanes 1–4, reagents alone at t = 0: Glu-Pg (lane 1), human plasmin (lane 2), fibrin (lane 3), and SUPA cut from the fusion partner maltose-binding protein (MBP; lane 4). Lanes 5–9, reagents after incubation at 37 °C for 0.5 h: Glu-Pg (lane 5), human plasmin (lane 6), Glu-Pg in the presence of fibrin (lane 7), Glu-Pg activated by SUPA (lane 8), and Glu-Pg activated by SUPA in the presence of fibrin (lane 9). Lane 10, protein standards and relative molecular mass (shown in kilodaltons).