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. 2012 Apr 19;287(23):19171–19176. doi: 10.1074/jbc.M112.359315

FIGURE 5.

FIGURE 5.

Activation of micro-Pg or micro-Pg(R561A) by different Pg activators. Human micro-Pg (uPg) or the cleavage-resistant mutant micro-Pg(R561A) (uPgR561A) was added to HEPES buffer containing 500 μm S-2251. After incubation for 10 min, 200 nm SK (A), 200 nm staphylokinase (B), 1000 nm SUPA (C), or no Pg activators were added to micro-Pg or micro-Pg(R561A) at an equimolar concentration. Controls consisted of micro-Pg (200 nm) or the cleavage-resistant mutant micro-Pg(R561A) (200 nm) in HEPES buffer containing 500 μm S-2251 without Pg activator. Results are representative of two experiments.