TABLE 2.
Effects of chloride and fibrin on activation of human Pg by SUPA
Activation experiments were carried out at 37 °C in a total volume of 100 μl with HEPES/NaCl or HEPES/NaOAc buffer in the absence or presence of 0.1 mg/ml fibrin (CNBr-fibrin fragments). The kinetic analysis of activation parameters is described under “Experimental Procedures.” Values represent the mean ± S.D.
| Buffer | Plasminogen activation parameters |
||
|---|---|---|---|
| KPg | kPg | kPg/KPg | |
| nm | min−1 | μm−1min−1 | |
| HEPES/NaCl | 202 ± 61 | 0.98 ± 0.02 | 4.8 |
| HEPES/NaOAc | 96 ± 15 | 3.52 ± 0.01 | 36.6 |
| HEPES/NaCl + fibrin | 48 ± 7 | 1.41 ± 0.01 | 29.6 |
| HEPES/NaOAc + fibrin | 30 ± 2 | 3.48 ± 0.01 | 114.3 |