FIGURE 3.

Pharmacological inhibition of IKKβ blocks type 1 interferon secretion in primary murine Flt3-derived dendritic cells. Murine bone marrow cells were incubated for 7 days with Flt3-ligand to obtain Flt3-derived dendritic cells. Cells were pretreated with 1 μm BI605906 and stimulated for the times indicated with 1 μg/ml of the TLR7 ligand CL097 (A) or for 6 h with 0.05 μm CpG type B or 1 μm CpG type A (B–D). RNA was extracted from the cells and mRNA encoding Ifnb (A and B), Ifna4 (C), and Ifna6 (D) was determined by quantitative PCR. The results are plotted as the fold-increase in mRNA relative to the level determined in unstimulated cells. The experiments were performed in 96-well plates with three wells being used for each condition, each containing Flt3-derived dendritic cells from a different mouse. The results are presented as the mean ± S.E. for one representative experiment. Similar results were obtained in two independent experiments.