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SodA expression is manganese-dependent. A, strain 297 was grown for 7 days in Chelex-treated BSK-II medium with no metal addition (lane 1), with 10 μm MnCl2 (lane 2), with 10 μm ZnSO4 (lane 3), or with 10 μm MnCl2 and 10 μm ZnSO4 (lane 4). B, strain B31-MI was grown as in A with no metal addition (lane 1), with 10 μm MnCl2 (lane 2), with 10 μm ZnSO4 (lane 3), or with 10 μm MnCl2 and 10 μm ZnSO4 (lane 4). The samples were probed for FlaB and SodA expression.
