FIGURE 6.

p38 inhibition is required for syndecan-2 expression. A, B16F10 cells treated with SB239063 (SB) described as Fig. 5D and mRNA level of syndecan-2 (sdc2) was analyzed by RT-PCR. β-Actin was used as the loading control (con, top panel). The cells (1.5 × 10⁵ cells) were harvested and seeded into upper chamber of Transwell plates. After 6 h, the migrated cells were stained with hematoxylin and eosin (bottom panel). **, p < 0.05 versus control. B, B6F10 cells were treated with the indicated amount of H₂O₂ in culture medium (1% FBS) for 24 h. Syndecan-2 mRNA expression was analyzed by RT-PCR, and the phosphorylation of p38 was analyzed by Western blotting with anti-phospho-specific p38 antibody (α-p-p38, top panel). Cell migration was analyzed as described for A (bottom panel). *, p < 0.01 versus 0 mm. C, B16F10 cells were pretreated with SB239063 (5 μm) for 30 min and then incubated an additional 24 h in the presence of H₂O₂ (2.5 mm). Syndecan-2 mRNA expression was analyzed by RT-PCR. The cells were lysed by radioimmune precipitation assay buffer and detected phospho-p38 (α-p-p38) by Western blotting (top panel). Cell mobility was measured by Transwell migration assay (bottom panel). **, p < 0.05 versus untreated cells.