Nucleotide gene and amino acid sequences of scleritin. Nucleotide sequence above (GenBank accession number JQ652458) and deduced translation of the ORF (nucleotides 40–507) below. The putative signal peptide is underlined, the peptide sequence obtained after Edman degradation is highlighted in gray, the two consecutive peptides obtained by MS/MS are indicated in bold letters, the potential N-glycosylation site is highlighted in black (Asp-152), the five potential O-glycosylation sites (Thr-24, Ser-56, Ser-57, Thr-150, and Ser-154) are indicated by asterisks, and the seven potential phosphorylation sites are boxed (Thr-24, Ser-65, Ser-72, Ser-73, Thr-113, Tyr-129, and Tyr-147).