FIGURE 6.

HJ9.3 traps RD(ΔK)-YFP aggregates at the cell surface. HEK293 cells were transfected with RD(ΔK)-YFP or were mock-transfected. HJ9.3 was added to the culture medium for the 48-h period. At the end of the experiment, the cells were fixed, permeabilized, and stained with an anti-mouse secondary antibody (labeled with Alexa 546). Confocal microscopy was used to analyze the localization of HJ9.3-Tau complexes. The top panels show that many complexes are identified when RD(ΔK)-YFP is expressed, but none are identified in its absence (bottom panels). Orthogonal analyses (right panel) demonstrate that most complexes are present at the cell surface, although occasional intracellular complexes were observed. NT, non-transfected; DIC, differential interference contrast.