FIGURE 3.

TRPV1 internalized in response to vanilloids is targeted to lysosomes. A and B, representative blot (IB) from cell surface biotinylation assays showing membrane (Memb) and total TRPV1 expression (upper panel) and total band density quantification (lower panel) of TRPV1⁺ cells exposed to vehicle (V) or 1 μm capsaicin in the absence or presence of 200 μg/ml chloroquine (Chlor) (A) or 75 μm leupeptin (Leup) (B) to inhibit lysosomal proteases. Data represent mean ± S.E., n = 3. **, p < 0.01. ns, not significant.