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. 2012 Apr 16;287(23):19516–19527. doi: 10.1074/jbc.M111.337428

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — PTTG1 regulates the breast cancer stem cell population. A, measurement of sphere sizes after treatment with shRNA targeted to PTTG1 for 72 h in BT549 cells. Sixty spheres per group were randomly taken to measure the size. B, clonal analysis of sphere-forming cells at single cell levels after treatment with shRNA targeting of PTTG1. C, quantification of breast cancer stem cell population by FACS. CD44^high CD24^low cell population was drastically increased in sphere-cultured conditions; however, treatment with shRNA targeting of PTTG1 suppressed CD44^high CD24^low cell population in sphere-cultured BT549 cells. D, treatment with shRNA targeting of PTTG1 decreased stemness-regulating transcription factor Sox2, but not Oct4 and Nanog. Error bars represent mean ± S.D. *, p < 0.001.