FIGURE 2.

Stat1 and Stat5 are not involved in G-CSF induced Bv8 up-regulation. A and B, Bv8 expression analysis in bone marrow cells incubated with Stat1 (A) or Stat5 inhibitor (B). Error bars represent S.E. Asterisks indicate significant difference between G-CSF and PBS treated groups (p < 0.05). n.s., not significant. C, phospho-Stat1, phospho-Stat5, and β-actin levels after G-CSF treatment. D, phospho-Stat1 following pretreatment with Stat 1 inhibitor for 4 h. E, phospho-Stat5 after pretreatment with inhibitor for 1 h at the indicated concentrations followed by incubation with G-CSF for an additional 10 min. Data shown are representative of three independent experiments.