FIGURE 1.

E signaling regulates Fra-1 expression during decidualization. A, mice were ovariectomized and subjected to artificially induced decidualization as described under “Experimental Procedures.” Cohorts of these animals were treated with progesterone alone (P) or P along with aromatase inhibitor (P + AI), and the uterine horns were separately collected after 72 h of stimulus (n = 5). Total RNA was isolated from these uterine samples, and real-time PCR analysis was performed using Fra-1- and Esr1-specific primers. B and C, stromal cells isolated from mice on day 4 of pregnancy were subjected to in vitro decidualization in the presence of increasing concentrations of aromatase inhibitor (AI) for 72 h. The RNA was analyzed by real-time PCR to monitor the expression of the Fra-1 and Esr1 transcript. Error bars, S.E.