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. 2012 Apr 18;287(23):19622–19630. doi: 10.1074/jbc.M111.297663

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Fra-1 is a critical regulator of mouse stromal cell decidualization. Primary mouse stromal cells transfected with Fra-1 and scrambled (NC) siRNA were collected 48 h after treatment. A, down-regulation of Fra-1 mRNA (upper) and protein (lower) was assessed by real-time PCR and immunocytochemistry, respectively. B, differential gene expression of decidualization markers such as alkaline phosphatase (ALP) and prolactin-related protein (PRP) in cells transfected with NC siRNA and Fra-1 siRNA was quantitated by real-time PCR. C, alkaline phosphatase activity in NC (left) and Fra-1 (right) siRNA-treated stromal cells. Error bars, S.E.