Skip to main content
. 2012 Apr 18;287(23):19622–19630. doi: 10.1074/jbc.M111.297663

FIGURE 6.

FIGURE 6.

Fra-1 regulates uterine stromal remodeling. Primary mouse stromal cells were transfected with Fra-1-specific and scrambled (NC) siRNAs. siRNA-transfected confluent monolayer of primary stromal cells were subjected to wound-healing assay to monitor cell migration. A, cells were recorded photographically under phase contrast microscopy at 0, 24, and 48 h following application of the wound. B, quantitative representation of stromal cell migration from five independent experiments is shown. *, p < 0.01; **, p < 0.001. C, cells were fixed after 48 h of the migration assay and subjected to immunocytochemical analysis using anti-Ki67 antibody. Proliferation of stromal cells as indicated by Ki-67 positive staining was similar in response to NC and Fra-1 siRNA treatments. D and E, relative level of gene expression of MMP2, MMP9, and MMP13 was analyzed by real-time PCR analysis. Error bars, S.E.