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Overview of CSP effects observed in β5-α1 loop region (i.e. Thr-89–Ile-96) relative to Bcl-2(1–211) R127Q/R129Q double mutant upon addition of 0.2 m ratio of wild-type Bcl-2(1–211) (black bars), Bcl-2(1–211) R127Q (gray bars), or Bcl-2(1–211) R129Q (white bars). The CSP effects were normalized to the respective shifts observed in the presence of wild-type Bcl-2.
