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. 2012 Apr 16;287(23):19674–19686. doi: 10.1074/jbc.M112.355503

FIGURE 6.

FIGURE 6.

Effect of sucrose concentration on Ffase mutant transferase activity. Enzymes (1 unit ml−1) and sucrose (250–1000 mm) were incubated for 3 h. The amount of carbohydrates formed or hydrolyzed/min was quantified by HPLC in the 1–3 h interval (initial velocity conditions). 6-Kestose was the only transfructosylation product detected in all reaction mixtures, and the amount of sucrose hydrolyzed was lower than 10% (w/w) in all cases.