TABLE 3.
FOS produced by Ffase mutants after 24-h reactions
Enzymes (1 unit ml−1) and 1 m sucrose were incubated for 24 h. FOS is indicated as g liter−1. ND, not detected.
| Ffase mutant | Neokestose | 1-Kestose | 6-Kestose | Total FOS | 6-K/1-K ratio |
|---|---|---|---|---|---|
| WT | ND | 9.5 | 33.4 | 42.9 | 3.5 |
| Q176S | ND | 5.3 | 21.1 | 26.4 | 3.9 |
| Q176N | ND | 6.6 | 24.7 | 31.3 | 3.7 |
| Q176E | ND | 6.1 | 18.9 | 25.0 | 3.1 |
| Q228V | 0.5 | 0.8 | 28.7 | 30.0 | 35.9 |
| Q228T | 1.0 | 2.6 | 3.3 | 6.9 | 1.3 |
| Q228N | 0.8 | 2.1 | 24.6 | 27.6 | 11.7 |
| Q228E | ND | 2.1 | 23.2 | 25.3 | 11.0 |
| N254A | 1.7 | 1.3 | 13.5 | 16.5 | 10.4 |
| N254T | 11.8 | 8.6 | 28.4 | 48.8 | 3.3 |
| N254D | 1.9 | 3.6 | 12.5 | 18.0 | 3.5 |
| N2254E | ND | 4.1 | 7.3 | 11.4 | 1.8 |