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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Genes Immun. 2012 Jan 5;13(4):299–310. doi: 10.1038/gene.2011.86

Figure 3. Ciita isoform I is not required for expression of CIITA or MHC-II in cells of the myeloid lineage.

Figure 3

Ciita isoform specific, total Ciita mRNA, or I-Aα mRNAs were quantified in Flt3L-elicited splenic DCs (left panels) or thioglycolate-elicited peritoneal macrophages (-/+IFN-γ) (right panels) were analyzed by qRT-PCR with the primer sets indicated. Flt3L elicited spDC were purified with CD11c+ MACs beads and were >95% CD11c+. Thioglycolate-elicited, peritoneal cavity macrophages were purified by adherence to tissue culture plates and were >95% CD11b+. A. Isoform specific PCR of CIITA in WT splenic dendritic cells (spDC) and macrophages treated for 24h with IFN-γ. B. Total Ciita mRNA levels in KI, KO, and WT littermate controls. C. I-Aα mRNA levels in KI, KO, and WT (littermate controls) spDC or macrophages treated with IFN-γ. D. Isoform specific expression of Ciita. HA-III represents primers specific for the Knock-in allele, HA-tagged isoform III derived from promoter I. Data were averaged from four mice for each genotype, with each representing one biological replicate. Significance was calculated using the students t-test. *p<0.05, **p<0.005.