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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Nat Cell Biol. 2012 May 13;14(6):593–603. doi: 10.1038/ncb2489

Figure 2. G2-specific Cdt1 inhibition induces mitotic arrest.

Figure 2

(a) HeLa cells synchronized in early S phase were released into control (luciferase) or cdt1 siRNA for either 9 hrs (top and middle panels) or 10 hrs (bottom panels) followed by fixation and staining with DAPI to label chromosomes (blue), anti-tubulin antibody to label MTs (red), and anti-Knl1 antibody to label kinetochores (green). (b) Quantification of the results at 10 hr in a by mitotic stage; n = 1500 cells. (c–e) HeLa cells stably expressing GFP-histone H2B were injected with control buffer (c, n=15) or anti-Cdt1 antibody (d, n=27). Selected frames of GFP-histone (top panels) and phase contrast images (bottom panels) are shown. (e) Quantification of the results from the microinjection experiments in c and d. Scale bars = 5 μm. See also Supplementary Movies S1–S4.