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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Comp Immunol Microbiol Infect Dis. 2012 Feb 12;35(4):289–301. doi: 10.1016/j.cimid.2012.01.010

Fig. 3. Representative dot plots (A) and mean frequencies (B) of IFN-γ producing γδ T cell subsets in ileum, spleen and blood of Gn pigs inoculated with HRV.

Fig. 3

MNCs were cultured in vitro for 17 hours without antigen stimulation before staining for flow cytometry analysis. The right two dot plots in panel (A) show the representatives of IFN-γ producing CD2+CD8+ subset in ileum of Gn pigs infected with HRV at PID 3 or mock infected. Figures in panel (B) compare the mean frequencies of IFN-γ producing γδ T cells among the three subsets in ileum, spleen and blood of Gn pigs infected with HRV (PID 3 and PID 5) or mock infected (PID 0). Data are presented as mean frequency ± standard error of the mean (n = 4–7). Different capital letters on top of bars indicate significant differences in frequencies among the time points for the same γδ T cell subset and tissue (Kruskal-Wallis test, p < 0.05), while shared letters indicate no significant difference.

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