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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Comp Immunol Microbiol Infect Dis. 2012 Feb 12;35(4):289–301. doi: 10.1016/j.cimid.2012.01.010

Fig. 5. Cytokine production by splenic and IEL γδ T cell subsets in cultures with or without IPP plus IL-2 stimulation.

Fig. 5

MNCs isolated from spleen and IEL of Gn pigs infected with human norovirus GII.4 were sorted into three γδ T cell subsets by using MACS and FACS technologies. Each γδ T cell subset was cultured in vitro for 5 days with the stimulation of IPP plus IL-2 or mock stimulated. Concentrations of IFN-γ, IL-10 and TGF-β in the culture supernatants were measured by ELISA. Data are presented as mean concentrations ± standard error of the mean (n = 6). Different capital letters on top of bars indicate significant differences in cytokine concentrations between IPP plus IL-2 stimulated and mock stimulated cells for the same γδ T cell subset and tissue (Kruskal-Wallis test, p < 0.05), while shared letters indicate no significant difference. Different numbers of * indicates significant difference in cytokine concentrations among the three γδ T cell subsets under the same stimulation condition for the same tissue (Kruskal-Wallis test, p < 0.05), while same numbers of * indicate no significant difference.

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