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. 2012 May 25;2(5):e73. doi: 10.1038/bcj.2012.17

Figure 6.

Figure 6

Percentage of viable CLL cells cocultured with hBM-MSCs in absence or presence of chemotherapeutic agents and neutralizing Notch receptor antibodies and GSI XII.Percentage of viable CLL cells (Annexin V−/7-AAD−, lower left quadrant) cultured alone in absence or presence of Fludarabine (5.0 μℳ) (a), Cyclophosphamide (100 μℳ) (b), Bendamustine (200.0 μℳ) (c), Prednisone (100.0 μℳ) (d) and Hydrocortisone (100.0 μℳ) (e), and cocultured with hBM-MSCs (10/1 ratio) in presence or absence of neutralizing Notch receptor antibodies or GSI XII for 3 days. CLL cells were analyzed by flow cytometry after electronic gating on CD5+CD19+CD45+ CLL cells. GSI XII was resuspended in dimethyl sulfoxide (DMSO) and DMSO was used as control for vehicle effects. One-way analysis of variance, Holm–Sidak test was used to compare the different groups. *P<0.05, **P<0.01, ***P<0.001 were considered statistically significant.