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. 2012 May 10;3(5):e306. doi: 10.1038/cddis.2012.42

Figure 1.

Figure 1

Bik mRNA levels are modestly increased in B-lymphoma cells and pre-malignant lymphoid cells from Eμ-Myc mice compared with control B-lymphoid cells. (a) Bik mRNA expression is increased in pre-neoplastic B-lymphoid cells and (b) in lymphoma cells from sick Eμ-Myc mice. Differences in the levels of mRNA for pro-apoptotic BH3-only genes between FACS-purified pre-B (B220+ sIgM−) and B (B220+ sIgM+) cell subsets obtained from healthy 4–6-week-old Eμ-Myc mice (a) or from sick lymphoma-bearing Eμ-Myc mice (b), compared with the corresponding non-transformed pre-B and B-lymphoid cells from age-matched control C57BL/6 mice. Relative RNA expression levels were calculated by normalising to the β-actin signal in each sample, then dividing the values from the Eμ-Myc transgenic B-lymphoid or -lymphoma cells by the value from the control (nontransgenic) cells (represented by the dotted horizontal line at 1). Data represent mean expression±S.E.M. of cells (lymphoma or control non-transformed) from three individual mice of each genotype analysed in three separate experiments. In pre-malignant Eμ-Myc B220+ sIgM+ B cells, the fold induction of Bik mRNA compared with that observed in control nontransgenic B220+ sIgM+ B cells, was significantly higher (*P<0.05) than the fold induction of Bad, Bid, Bim and Noxa