Figure 2.

Akt protects from Ca²⁺-dependent apoptosis in COS7, but not in SH-SY 5Y cells. (a) COS7 cells were transfected with the indicated gene (control: mock-transfected), harvested after 36 h, and the endogenous cleavages of PARP and caspase 3 were revealed through immunoblotting. Apoptosis was induced by treatment with 80 μM AA for 40 min. (b) COS7 cells were transfected and treated in the same manner of a, aliquots of cells were centrifuged, and lysates were assayed for caspase 3 activity as described in Materials and Methods. Traces are from a single representative experiment. (c) Immunoblotting showed different levels of cleaved PARP and caspase 3 in SH-SY 5Y after apoptosis induction and m/p-Akt overexpression. control, mock transfected. (d) Similar experiment to b but performed in SH-SY 5Y cells. The experiments were repeated at least five times