Figure 3.

Akt modulates [Ca²⁺]_c elevation generated by AA. (a) Effects of m/p-Akt on [Ca²⁺]_c increase induced by AA in COS7 or (b) in SH-SY 5Y cells. Cells were loaded with the Ca²⁺ indicator Fura-2/AM and the kinetics of the [Ca²⁺]_c response is presented as the ratio of fluorescence at 340 and 380 nm. The coverslips with the cells were maintained in 1 mM Ca²⁺/KRB and, where indicated, the cells were challenged with 80 μM AA. To distinguish Akt-positive cells and compare changes in the 340/380 Fura-2/AM ratio on the same coverslip, cells were co-transfected with mtGFP and m/p-Akt in a 1 : 1 ratio. Traces are from a single representative experiment. The data are the mean of different angular coefficients±S.E. of, at least, 15 independent experiments; the bars in the graph show the change in percentage of cytosolic Ca²⁺ released