Figure 7.

MPP⁺ inhibits synthesis of mtDNA-encoded respiratory subunits, restored by U0126. A representative autoradiogram showed that MPP⁺ treatment for 1 week caused marked decreases in newly synthesized ³⁵S-Met-labeled mtDNA-encoded translation products, reversed by co-treatment with U0126 (a). In contrast, overall protein translation levels assessed in the absence of emetine revealed no obvious effects on total (predominantly nuclear-encoded) protein synthesis (b). The graph (c) is a quantification of three independent pulse-labeling experiments normalized by Coomassie Brilliant Blue stain. **P<0.01 MPP⁺ versus Ctrl, Ctrl+UO, MPP⁺+UO. ^†P<0.05 Ctrl+UO or MPP⁺+UO versus Ctrl