Figure 5.
Actin stabilization by p57KIP2 promotes displacement of HK-1 from mitochondria. (a) HeLa-p57KIP2 cells were treated with either Dox or Jasp. Mitochondrial and cytosolic protein fractions were analyzed by immunoblotting using antibodies against HK-1. Tom40 was used as a mitochondrial loading control. Asterisk (*) indicates a cleavage product of HK-1. Values represent the fold decrease of full-length HK-1 compared with control. (b) HeLa-p57KIP2 cells were transfected with HK-1-GFP or pcDNA and grown in the presence or absence of Dox for 24 h. Overexpression of HK-1-GFP was analyzed by immunoblotting with GFP, using Tom40 as a loading control, and (c) cleaved caspase-3 staining was assessed by flow cytometry